Protein_Domain

Part:BBa_J70549:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-15)

GFP C-Terminal fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 430


Design Notes

designed


Source

designed

PCRd from E0040 using

Forward primer (J70549-f): gtttcttcgaattcgcggccgcttctagagcgagatacccagatcatatgaaacagc

Reverse primer (J70549-r): tttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

References